Purification of simian virus 40 large T antigen by immunoaffinity chromatography

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Partial purification of Simian virus 40 large T antigen by immunoaffinity chromatography and its detection by enzyme-linked immunosorbent assay.

Simian virus 40 (SV40) large T antigen was partially purified from small amounts of SV40-infected and SV40-transformed cells by immunoaffinity chromatography with high recovery. T antigen, in both crude and partially purified states, was detected rapidly by a sensitive and quantitative enzyme-linked immunosorbent assay (ELISA). Stability of the partially purified T antigen was found to increase...

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Simian virus 40 large T antigen binds to topoisomerase I.

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Association of simian virus 40 T antigen with simian virus 40 nucleoprotein complexes.

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1985

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.53.3.1001-1004.1985